The Eye-Bank's Patricia Dahl Ocular Tissue Research Program provides quality donor eye tissue to support advancements in ocular research. The program provides customized “control” and diseased ocular research tissues to assist with ocular, neurodegenerative and metabolic studies.
Fully Equipped Ocular Laboratory
- Biosafety cabinet 2 (BSC2)
- Dissection microscope
- Light microscope with camera
- CO2 incubator
- Konan CellCheck D+ specular microscope
- Slit lamp biomicroscope
- Zeiss Cirrus 5000 OCT machine
- Lumedica Retina and Cornea OCT Microscope
- Applied Biosystem PCR Thermal Cycler
Research Tissue Available
Tissues
- Whole eye
- Posterior pole
- Iris
- Lens
- Retina
- Conjunctiva
Primary adult ocular cells*
- Adult human primary retina pigmented epithelial cells (ahRPE-sc)
- Adult human primary Müller cells
Tissue processing*
- RNA, DNA and Protein extraction
- Dissection epithelium or endothelium layer of cornea
Phase contrast image of RPE cell
Photo Credit: The Eye-Bank for Sight Restoration
AhRPE cells isolated from human cadaveric tissue (DIV 30), fixed and immunostained for RPE markers (Scale bar = 20μm.)
Photo Credit: The Eye-Bank for Sight Restoration
Cornea processing-dissection of Descemet membrane and endothelium
Photo Credit: The Eye-Bank for Sight Restoration* Customization available upon request
Preservation Methods
Fixatives*
- Formalin / formaldehyde
- Davidson's fixative
- Hartmann's fixative
- RNAlater
- 8M Urea
- Paraformaldehyde (PFA)
Media*
- Optisol-GS
- DMEM
- Hybernation media
Other*
- Moist chamber
- Flash freezing (LN2)
- Freezing in OCT (optimal cutting temperature) compound
* Customization available upon request
Collaborate With Our Team
The Eye-Bank for Sight Restoration collaborates with researchers to optimize research protocols; study design; grant proposal writing, manuscript writing and more.